Evaluation of Surrogate Tests for the Presence of mecA -Mediated Methicillin Resistance in Staphylococcus capitis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus warneri

epidermidis, ATCC 15305 Staphylococcus saprophyticus, ATCC 35663 Staphylococcus xylosus, ATCC 27840 Staphylococcus capitis subsp. capitis, ATCC 43809 Staphylococcus lugdunensis, ATCC 29970 Staphylococcus haemolyticus, ATCC 27844 Staphylococcus hominis subsp. hominis, and ATCC 43300 methicillin-resistant S. aureus (MRSA). Clinical isolates

Homology of mecA gene in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans to that of Staphylococcus aureus.

A penicillin-binding protein of molecular weight 76,000 inducible by beta-lactams was detected in methicillin-resistant Staphylococcus haemolyticus and Staphylococcus simulans. DNA from these strains hybridized to the mecA gene from Staphylococcus aureus; however, the chromosomal HindIII fragments containing the mecA genes were 3.4 kilobases in S. haemolyticus and 4.3 kilobases in S. simulans.

Additional Penicillin-Binding Protein in Methicillin- Resistant Staphylococcus epidermidis, Staphylococcus haemolyticus, Staphylococcus hominis, and Staphylococcus simulans with a Low Affinity for Methicillin, Cephalothin, and Cefamandole

The presence of an additional penicillin-binding protein (PBP) was demonstrated in methicillin-resistant strains of Staphylococcus epidermidis, S. haemolyticus, S. hominis, and S. simulans. In these four species, the apparent molecular mass of this protein was analogous to that of PBP 2' of methicillin-resistant S. aureus SR 1550-9. It exhibited a low affinity for methicillin, cephalothin, and ...

Prevalence of Vancomycin Resistance in Methicillin-Resistant Staphylococcus Aureus

Background & Aims: Staphylococcus aureus is one of the most common pathogens in nosocomial infections. Vancomycin is the most important therapeutic drug of choice for treatment of infections caused by methicillin-resistant Staphylococcus aureus (MRSA) strains. Therefore, vancomycin-resistant Staphylococcus aureus (VRSA) or vancomycin-intermediate Staphylococcus aureus (VISA) strains are warning...

A study of coagulase-negative staphylococci (CoNS) isolated from bovine mastitis for the presence of penicillin and methicillin resistance-encoding genes in the north west of Iran

Coagulase-negative staphylococci (CoNS) are often associated with bovine mastitis and may be resistant to antimicrobial therapy. The aim of the current study was to investigate the presence of blaZ (responsible for penicillin resistance) and mecA (responsible for methicillin resistance) genes among 108 CoNS belonging to 9 different species isolated from bovine mastitis in seven dairy herds (H1-...